Bacterial community structure in simultaneous nitrification, denitrification and organic matter removal process treating saline mustard tuber wastewater as revealed by 16S rRNA sequencing. (March 2017)

• Record Type: Journal Article
• Authors: Wang, Jiale; Gong, Benzhou; Huang, Wei; Wang, Yingmu; Zhou, Jian
• Is Part Of: Bioresource technology. Volume 228(2017)
• Page Start: 31
• Page End: 38
• Publication Date: 2017-03
• Subjects: Microbial community -- Full-length 16S rRNA sequencing -- Saline mustard tuber wastewater -- Archaea -- Partial nitrification and denitrification; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.12.071
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: First systematically investigated SNDOR treating MTWW by full-length 16S rRNA sequencing. Proliferation of halophilic microbes stabilized biological performance along with elevated salinity. Autotrophic AOB and AOA together contributed to ammonium oxidation despite of high C/N. PND gradually took predominance along with elevated salinity. Abstract: A simultaneous nitrification, denitrification and organic matter removal (SNDOR) process in sequencing batch biofilm reactor (SBBR) was established to treat saline mustard tuber wastewater (MTWW) in this study. An average COD removal efficiency of 86.48% and total nitrogen removal efficiency of 86.48% were achieved at 30 g NaCl L −1 during 100 days' operation. The underlying mechanisms were investigated by PacBio SMRT DNA sequencing (V1–V9) to analyze the microbial community structures and its variation from low salinity at 10 g NaCl L −1 to high salinity at 30 g NaCl L −1 . Results showed elevated salinity did not affect biological performance but reduced microbial diversity in SBBR, and halophilic bacteria gradually predominated by succession. Despite of high C/N, autotrophic ammonia-oxidizing bacteria (AOB) Nitrosomonas and ammonia-oxidizing archaea (AOA) Candidatus Nitrososphaera both contributed to ammonium oxidation. As salinity increasing, nitrite-oxidizing bacteria (NOB) were significantly inhibited, partial nitrification and denitrification (PND) process gradually contributed to nitrogen removal.