Effects of experimental left varicocele repair on hypoxia‐inducible factor‐1α and vascular endothelial growth factor expressions and angiogenesis in rat testis. (14th May 2016)

• Record Type: Journal Article
• Authors: Goren, M. R.; Kilinc, F.; Kayaselcuk, F.; Ozer, C.; Oguzulgen, I.; Hasirci, E.
• Is Part Of: Andrologia. Volume 49:Number 2(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2016-05-14
• Subjects: HIF‐1α protein -- hypoxia -- varicocele -- varicocelectomy -- vascular endothelial growth factor; Andrology -- Periodicals; Infertility, Male -- Periodicals; 616.69
• DOI: 10.1111/and.12614
• Languages: English
• ISSNs: 0303-4569

Abstract

Summary: This study was aimed to investigate the effects of experimental left varicocele (ELV) repair on hypoxia‐inducible factor‐1α (HIF‐1α) and vascular endothelial growth factor (VEGF) expressions and angiogenesis in rat testis. ELVs were surgically created in 26 adult male Sprague‐Dawley rats. Thirty days after surgery, ELV repair was performed in 13 of the rats. All rats subsequently underwent orchiectomy 30 days after the last laparotomy. Histology of ELV‐repaired testicles was compared to that of the unrepaired (ELV) group. The frequency of positive HIF‐1α findings was significantly lower in the ELV‐repaired than in the ELV group. The frequency of positive VEGF findings was also lower in the ELV‐repaired than in the ELV group, although the difference was not statistically significant ( P = 0.238). The mean microvessel density in ELV‐repair group was significantly lower than that in the ELV group ( P = 0.002). Our study demonstrated that ELV repair may protect tissues from hypoxia and hypoxia‐related pathophysiologic events, such as angiogenesis, in rat testis.