Genetic variability of the stable fly assessed on a global scale using amplified fragment length polymorphism. (19th May 2015)

• Record Type: Journal Article
• Authors: Kneeland, Kathleen M.; Skoda, Steven R.; Foster, John E.
• Is Part Of: Insect science. Volume 23:Number 5(2016)
• Page Start: 695
• Page End: 703
• Publication Date: 2015-05-19
• Subjects: AFLP -- biting flies -- external parasites -- genetic diversity -- livestock pests -- Stomoxys calcitrans; Insects -- Periodicals; Entomology -- Periodicals; 595.705
• DOI: 10.1111/1744-7917.12219
• Languages: English
• ISSNs: 1672-9609

Abstract

Abstract: The stable fly, Stomoxys calcitrans (L.) (Diptera: Muscidae), is a blood‐feeding, economically important pest of animals and humans worldwide. Improved management strategies are essential and their development would benefit from studies on genetic diversity of stable flies. Especially if done on a global scale, such research could generate information necessary for the development and application of more efficient control methods. Herein we report on a genetic study of stable flies using amplified fragment length polymorphism, with samples of 10–40 individuals acquired from a total of 25 locations in the Nearctic, Neotropic, Palearctic, Afrotropic and Australasian biogeographical regions. We hypothesized that genetic differentiation would exist across geographical barriers. Although F ST (0.33) was moderately high, the G ST (0.05; representing genetic diversity between individuals) was very low; N m values (representing gene flow) were high (9.36). The mismatch distribution and tests of neutrality suggested population expansion, with no genetic differentiation between locations. The analysis of molecular variance (AMOVA) results showed the majority of genetic diversity was within groups. The mantel test showed no correlation between geographic and genetic distance; this strongly supports the AMOVA results. These results suggest that stable flies did not show genetic differentiation but are panmictic, with no evidence of isolation by distance or across geographical barriers.