Dereplication of cytotoxic compounds from different parts of Sophora pachycarpa using an integrated method of HPLC, LC-MS and 1H-NMR techniques. Issue 11 (3rd June 2017)

• Record Type: Journal Article
• Authors: Shour, Sara; Iranshahy, Milad; Pham, Ngoc; Quinn, Ronald J.; Iranshahi, Mehrdad
• Is Part Of: Natural product research. Volume 31:Issue 11(2017)
• Page Start: 1270
• Page End: 1276
• Publication Date: 2017-06-03
• Subjects: Sophora pachycarpa -- alopecurone A -- flavonostilbene -- cytotoxicity; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2016.1239095
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: Sophora pachycarpa Schrenk ex C.A.Mey. is an annual plant belonging to the family Fabaceae. The cytotoxic activities of methanol-dichloromethane extracts (1:1) of different parts of S. pachycarpa were investigated on DU145 (prostate cancer cell line) and MCF-7 (breast cancer cell line) cell lines. The root extract of S. pachycarpa was the only extract that showed significant cytotoxic activity with IC50 values of 39.88 and 16.49 μg/mL on DU145 and MCF-7 cell lines, respectively. The root extract was then subjected to RP-HPLC for further fractionations. Among the isolated fractions from root extract, only one of them had remarkable cytotoxic effects with IC50 value of 26.43 on MCF-7 and 7.54 μg/mL on DU145 cell lines. Further purification led to isolation of a compound with IC50 values of 5.44 and 2.44 μg/mL on MCF-7 and DU145 cell lines, respectively. Based on 1 H NMR and 13 C NMR spectra, together with LC-MS, the structure of the purified compound was assigned as the flavonostilbene alopecurone A. Abstract :