A field evaluation of an isothermal DNA amplification assay for the detection of Theileria annulata infection in cattle. (February 2017)

• Record Type: Journal Article
• Authors: Gomes, Jacinto; Santos, Marcos; Amaro, Ana; Pereira da Fonseca, Isabel; Santos-Gomes, Gabriela; Inácio, João
• Is Part Of: Molecular and cellular probes. Volume 31(2017)
• Page Start: 61
• Page End: 64
• Publication Date: 2017-02
• Subjects: Molecular diagnostics -- LAMP -- Real-time PCR -- Tams1 -- Theileria annulata -- Bovine theileriosis; Molecular probes -- Diagnostic use -- Periodicals; Pathology, Cellular -- Technique -- Periodicals; Cell Biology -- Periodicals; Molecular Biology -- Periodicals; Sondes moléculaires -- Utilisation diagnostique -- Périodiques; Cytopathologie -- Technique -- Périodiques; 572
• DOI: 10.1016/j.mcp.2016.12.006
• Languages: English
• ISSNs: 0890-8508

Abstract

Abstract: A loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Theileria annulata infection in cattle. The results were compared with a real-time PCR used for the quantification of T. annulata parasitaemia. One hundred bovine blood samples from 16 cattle farms were tested with LAMP and real-time PCR, with T. annulata DNA being detected in 66% and 67% of the samples, respectively. The results showed that the LAMP assay detects a parasitaemia as low as 0.00025%, indicating a high analytical sensitivity of LAMP for clinical diagnosis of bovine theileriosis. Highlights: A LAMP assay for specific detection of Theileria annulata in cattle was evaluated in a field trial. A detection of a 0.00025% parasitaemia was achieved. The assay is useful for simple and rapid detection of T. annulata from clinical samples.