Application of Goldmag immune probe in timely detection of syphilis based on GIS platform. (3rd April 2017)

• Record Type: Journal Article
• Authors: Tang, Zhong; Liang, Zhisheng; Nong, Yi; Wu, Xiaochun; Luo, Hui; Gao, Kun
• Is Part Of: Artificial cells, nanomedicine, and biotechnology. Volume 45:Number 3(2017)
• Page Start: 460
• Page End: 466
• Publication Date: 2017-04-03
• Subjects: Geographic information system -- goldmag immunoprobes -- goldmag particles -- point-of-care testing; Artificial cells -- Periodicals; Nanotechnology -- Periodicals; Blood substitutes -- Periodicals; Tissue engineering -- Periodicals; Molecules -- Periodicals; Biotechnology -- Periodicals; 615.39
• DOI: 10.3109/21691401.2016.1170695
• Languages: English
• ISSNs: 2169-1401

Abstract

Abstract: The purpose of this study was to apply goldmag immunoprobes into establishment of nanoparticles-based colorimetric assay as well as construction of immunochromatography quantitative and qualitative system by exploring point-of-care testing of syphilis with goldmag particles carrier-based immunoprobe and analysis of spatial data of Geographic Information System (GIS) platform. Goat anti-rabbit immunoglobulin G (IgG) was coupled on the surface of modified nanoparticles, taking N-(3-dimethylaminopropyl)-N'-ethyl-carbodiimide as the connector. Then the nanoparticles were used for colorimetric detection of goat-anti-rabbit IgG in liquid phase system. Based on the analysis of spatial data in GIS platform, we found the probe constructed based on MUA-Fe304/Au nanoparticles responded more sensitive to detection objects compared with the probe designed based on PAA-Fe3 O4 /Au nanoparticles, and its reaction rate constant was two times that of PAA-Fe3 O4 /Au nanoparticles based goldmag immunoprobe. Goldmag particles not only can be coupled with biomolecules such as antibody/antigen and glycoprotein but also possess superparamagnetism.