Ethanolic Extract of Alternanthera sessilis (AS-1) Inhibits IgE-mediated Allergic Response in RBL-2H3 Cells. (August 2013)

• Record Type: Journal Article
• Authors: Rayees, Sheikh; Kumar, Ajay; Rasool, Shafaq; Kaiser, Peerzada; Satti, Naresh Kumar; Sangwan, Payare Lal; Singh, Surjeet; Johri, Rakesh Kamal; Singh, Gurdarshan
• Is Part Of: Immunological investigations. Volume 42:Number 6(2013)
• Page Start: 470
• Page End: 480
• Publication Date: 2013-08
• Subjects: Alternanthera sessilis -- RBL-2H3 cells -- IL-6 -- TNF-α -- IL-13 -- IL-4 -- NF-κB -- IκBα -- β-hexoaminidase.; Immunology -- Periodicals; Immunochemistry -- Periodicals; Cellular immunity -- Periodicals; Communicable diseases -- Periodicals; 616.079
• DOI: 10.3109/08820139.2013.789909
• Languages: English
• ISSNs: 0882-0139

Abstract

Abstract : The present study was designed to investigate the anti-allergic effects of ethanolic extract of Alternanthera sessilis (AS-1) in rat basophilic leukemia (RBL-2H3) cells. It significantly reduced the β-hexosaminidase release from anti-DNP-IgE sensitized RBL-2H3 cells. AS-1also inhibited the IgE antibody-induced increase in Interleukin-6 (IL-6), TNF-α, IL-13 and IL-4 production in these cells. The inhibitory effect of AS-1 on these cytokine was found to be nuclear factor-KB (NF-kB) dependent, as it attenuated the degradation of IKBa and nuclear translocation of NFkB. In addition, AS-1 significantly attenuated the DNP HAS-induced intracellular Ca 2+ release from these cells, which makes us speculate strongly that the decreased intracellular Ca 2+ is involved in the inhibitory effect of AS-1 on β-hexoaminidase release. Taken together, anti-allergic effects of AS-1 suggest possible therapeutic application of this extract in allergic diseases.