Hollow carbon spheres trigger inflammasome-dependent IL-1β secretion in macrophages. (March 2017)

• Record Type: Journal Article
• Authors: Andón, Fernando T.; Mukherjee, Sourav P.; Gessner, Isabel; Wortmann, Laura; Xiao, Lisong; Hultenby, Kjell; Shvedova, Anna A.; Mathur, Sanjay; Fadeel, Bengt
• Is Part Of: Carbon. Volume 113(2017)
• Page Start: 243
• Page End: 251
• Publication Date: 2017-03
• Subjects: ASC apoptosis-associated speck-like protein -- CBMs carbon-based nanomaterials -- HCS hollow carbon spheres -- HMDM human monocyte-derived macrophages -- LAL limulus amoebocyte lysate -- LPS lipopolysaccharide -- NAC N-acetyl-cysteine -- NLRP3 NLR (nucleotide-binding domain and leucine-rich repeat containing) family pyrin domain containing 3 (also known as NALP3 -- for NACHT LRR and PYD domain-containing protein 3); Carbon -- Periodicals; Carbone -- Périodiques; Koolstof; Toepassingen; Electronic journals; 546.681
• DOI: 10.1016/j.carbon.2016.11.049
• Languages: English
• ISSNs: 0008-6223

Abstract

Abstract: It is disputed whether inflammasome activation leading to secretion of pro-inflammatory interleukin (IL)-1β in macrophages transpires independently of cell death or whether the two processes are linked. Here, we synthesized hollow carbon spheres (HCS) and investigated their effects on primary human monocyte-derived macrophages (HMDM); short (500 nm) non-functionalized single-walled carbon nanotubes (SWCNT) were included for comparison. HCS (250 nm) were readily taken up by HMDM and induced ROS production, but did not trigger a loss of cell viability. However, a dose- and time-dependent release of IL-1β was detected in lipopolysaccharide (LPS)-primed macrophages upon exposure to HCS, while SWCNT-induced secretion of IL-1β was less pronounced. HCS-triggered IL-1β secretion was cathepsin B- and caspase-1-dependent, and was accompanied by a reduction in intracellular K + . Furthermore, cytokine secretion was reduced following treatment with the antioxidant, N -acetylcysteine, and cytochalasin D, an inhibitor of actin polymerization. HCS also triggered IL-1β release in LPS-primed THP.1 cells, but not in THP.1 cells with silencing of ASC, NLRP3, or caspase-1 expression, providing evidence that IL-1β was elicited through NLRP3 inflammasome activation. These studies shed light on the effects of HCS on primary macrophages, and show that spherical carbon-based nanoparticles are potent inflammasome activators. Graphical abstract: