Serum levels and activity of indoleamine2, 3-dioxygenase and tryptophanyl-tRNA synthetase and their association with disease severity in patients with chronic kidney disease. (7th May 2013)

• Record Type: Journal Article
• Authors: Bao, Yu-Shi; Ji, Ying; Zhao, Shi-Lei; Ma, Lin-Lin; Xie, Ru-Juan; Na, Shi-Ping
• Is Part Of: Biomarkers. Volume 18:Number 5(2013)
• Page Start: 379
• Page End: 385
• Publication Date: 2013-05-07
• Subjects: Chronic kidney disease -- immune regulation -- indoleamine2, 3-dioxygenase -- tryptophanyl-tRNA synthetase; Biochemical markers -- Periodicals; 610.28
• DOI: 10.3109/1354750X.2013.790074
• Languages: English
• ISSNs: 1354-750X

Abstract

Abstract: Objective : This study aims to test the serum levels and activity of indoleamine2, 3-dioxygenase(IDO) and tryptophanyl-tRNA synthetase (TTS) in patients with chronic kidney disease (CKD) and to evaluate their association with disease severity. Method : Serum concentrations of IDO and TTS in 61 patients with CKD and 16 healthy volunteers were tested by ELISA. Tryptophan and kynurenine concentrations were measured by high-performance liquid chromatography (HPLC). Results : Patients with CKD showed higher serum levels of IDO and TTS in comparison to healthy controls ( p = 0.001). Patients with CKD showed lower serum levels of tryptophan and higher serum levels of kynurenine in comparison to healthy controls ( p < 0.001). The kyn/Trp ratio significantly correlated with the disease severity in CKD patients ( r = 0.721; p < 0.001). Conclusions : IDO and TTS may play critical roles in the immune pathogenesis of CKD. The activity of IDO correlated with the disease severity of CKD.