GPER Promoter Methylation Controls GPER Expression in Breast Cancer Patients. (7th February 2017)

• Record Type: Journal Article
• Authors: Weissenborn, Christine; Ignatov, Tanja; Nass, Norbert; Kalinski, Thomas; Dan Costa, Serban; Zenclussen, Ana Claudia; Ignatov, Atanas
• Is Part Of: Cancer investigation. Volume 35:Number 2(2017)
• Page Start: 100
• Page End: 107
• Publication Date: 2017-02-07
• Subjects: GPER -- Promoter methylation -- Breast cancer -- GPR30; Cancer -- Periodicals; Oncology -- Periodicals; Medical Oncology -- Periodicals; Neoplasms -- Periodicals; 616.994
• DOI: 10.1080/07357907.2016.1271886
• Languages: English
• ISSNs: 0735-7907

Abstract

ABSTRACT: Recently, we found that G-protein-coupled estrogen receptor (GPER) protein expression decreased during breast carcinogenesis, and that GPER promoter is methylated. Here we analyzed GPER promoter methylation in 260 primary breast cancer specimens by methylation-specific polymerized chain reaction. The results demonstrated that GPER protein down-regulation significantly correlated with GPER promoter hypermethylation ( p < .001). Comparison of 108 tumors and matched normal breast tissues indicated a significant GPER down-regulation in cancer tissues correlating with GPER promoter hypermethylation ( p < .001). The latter was an unfavorable factor for overall survival of patients with triple-negative breast cancer ( p = .025). Thus GPER promoter hypermethylation might be used as a prognostic factor.