Overexpression of Withania somnifera SGTL1 gene resists the interaction of fungus Alternaria brassicicola in Arabidopsis thaliana. (January 2017)

• Record Type: Journal Article
• Authors: Mishra, Manoj K.; Srivastava, M.; Singh, G.; Tiwari, S.; Niranjan, A.; Kumari, N.; Misra, P.
• Is Part Of: Physiological and molecular plant pathology. Volume 97(2017:Jan.)
• Page Start: 11
• Page End: 19
• Publication Date: 2017-01
• Subjects: Glycosylation -- Alternaria brassicicola -- Overexpressed lines -- Chlorophyll fluorescence -- Phenolics; A. brassicicola Alternaria brassicicola -- Dpi Days post inoculation -- hpi Hours post inoculation -- MeJA Methyl jasmonate -- NPQ Non photochemical quenching -- LOX2 Lipoxygenase 2 -- PAD3 Phytoalexin deficient 3 -- SA Salycilic acid -- SGT Sterol glycosyltransferase -- Y (II) Yield; Plant diseases -- Periodicals; Diseased plants -- Physiology -- Periodicals; Phytopathogenic microorganisms -- Host plants -- Periodicals; 632
• DOI: 10.1016/j.pmpp.2016.11.003
• Languages: English
• ISSNs: 0885-5765

Abstract

Abstract: Heterologus transformed Arabidopsis plants improved tolerance against abiotic stress by the modulation of glycosylation of sterols and sterol glycosides. It is due to enhanced activity of Ws SGTL1 enzyme in different stresses. Presently, the effect of WsSGTL1 gene was investigated, focusing on interaction of A. brassicicola fungus on transgenic lines. Overexpressed lines showed restricted lesion, less spore counts and few electrolyte leakages. Biochemical study suggested that the transgenic lines had more glycosylated sterol/phenolic compounds. Relative expression of JA biosynthesis gene and phenylalanine ammonia lyase ( PAL) showed enhanced expression. Photosynthesis and chlorophyll fluorescence imaging revealed that transgenic plants had minimum damage of photosynthetic apparatus because of minimum PSII destruction. Graphical abstract: Highlights: WsSGTL1 overexpressed lines had shown restricted lesion diameter, less spore counts and few electrolyte leakages. HPLC–PDA analysis resulted overexpressed lines have comparatively more glycosylated sterol/phenolic compounds. Relative expression defense marker genes showed enhanced expression at earlier time period. PAL gene showed relative expression at later time period in transgenic lines. Chlorophyll fluorescence imaging also revealed that overexpressed lines had minimum damage of photosynthetic apparatus.