Fluorinated trimethyllysine as a 19F NMR probe for trimethyllysine hydroxylase catalysis. Issue 6 (24th January 2017)

• Record Type: Journal Article
• Authors: Vijayendar Reddy, Y.; Al Temimi, Abbas H. K.; Mecinović, Jasmin
• Is Part Of: Organic & biomolecular chemistry. Volume 15:Issue 6(2017)
• Page Start: 1350
• Page End: 1354
• Publication Date: 2017-01-24
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c6ob02683d
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : Human trimethyllysine hydroxylase (TMLH)-catalysed C-3 hydroxylation of N ε -(fluoromethyl)dimethyllysine can be monitored by 19 F NMR spectroscopy. Abstract : Trimethyllysine hydroxylase (TMLH) catalyses C-3 hydroxylation of N ε -trimethyllysine in the first step of carnitine biosynthesis in humans. Studies on TMLH have been hampered by the lack of established chemical methods. We report that an N ε -trimethyllysine analogue that contains the fluoromethyl group can be used as a 1 H and 19 F NMR probe for studies on TMLH catalysis.