Recent advances in high-throughput 13C-fluxomics. (February 2017)

• Record Type: Journal Article
• Authors: Heux, Stéphanie; Bergès, Cécilia; Millard, Pierre; Portais, Jean-Charles; Létisse, Fabien
• Is Part Of: Current opinion in biotechnology. Volume 43(2017)
• Page Start: 104
• Page End: 109
• Publication Date: 2017-02
• Subjects: Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.copbio.2016.10.010
• Languages: English
• ISSNs: 0958-1669

Abstract

Graphical abstract: Highlights: 13 C-fluxomics provides quantification of metabolic activity in microorganisms. Increasing flux analysis capacity has the potential to accelerate strain design. HT 13 C-fluxomics represents a trade-off between throughput and resolution. HT workflows require automation, parallelization and integration of each step. Recent advances may boost the emergence of integrated HT 13 C-fluxomics workflows. Abstract : The rise of high throughput (HT) strain engineering tools accompanying the area of synthetic biology is supporting the generation of a large number of microbial cell factories. A current bottleneck in process development is our limited capacity to rapidly analyze the metabolic state of the engineered strains, and in particular their intracellular fluxes. HT 13 C-fluxomics workflows have not yet become commonplace, despite the existence of several HT tools at each of the required stages. This includes cultivation and sampling systems, analytics for isotopic analysis, and software for data processing and flux calculation. Here, we review recent advances in the field and highlight bottlenecks that must be overcome to allow the emergence of true HT 13 C-fluxomics workflows.