Multidimensional NMR approaches towards highly resolved, sensitive and high-throughput quantitative metabolomics. (February 2017)

• Record Type: Journal Article
• Authors: Marchand, Jérémy; Martineau, Estelle; Guitton, Yann; Dervilly-Pinel, Gaud; Giraudeau, Patrick
• Is Part Of: Current opinion in biotechnology. Volume 43(2017)
• Page Start: 49
• Page End: 55
• Publication Date: 2017-02
• Subjects: Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.copbio.2016.08.004
• Languages: English
• ISSNs: 0958-1669

Abstract

Graphical abstract: Highlights: Multidimensional NMR is a powerful tool for complex samples in metabolomics. Tailored 2D NMR strategies enable the accurate quantification of metabolites. Fast 2D NMR methods are compatible with high-throughput applications. Hyperpolarisation methods have the potential to boost the sensitivity of NMR. Abstract : Multi-dimensional NMR is an appealing approach for dealing with the challenging complexity of biological samples in metabolomics. This article describes how spectroscopists have recently challenged their imagination in order to make 2D NMR a powerful tool for quantitative metabolomics, based on innovative pulse sequences combined with meticulous analytical chemistry approaches. Clever time-saving strategies have also been explored to make 2D NMR a high-throughput tool for metabolomics, relying on alternative data acquisition schemes such as ultrafast NMR. Currently, much work is aimed at drastically boosting the NMR sensitivity thanks to hyperpolarisation techniques, which have been used in combination with fast acquisition methods and could greatly expand the application potential of NMR metabolomics.