Dual colorimetric and fluorogenic probes for visualizing tyrosine phosphatase activity. Issue 14 (1st February 2017)

• Record Type: Journal Article
• Authors: Biswas, Suvendu; McCullough, Brandon S.; Ma, Elena S.; LaJoie, Dollie; Russell, Colin W.; Garrett Brown, D.; Round, June L.; Ullman, Katharine S.; Mulvey, Matthew A.; Barrios, Amy M.
• Is Part Of: Chemical communications. Volume 53:Issue 14(2017)
• Page Start: 2233
• Page End: 2236
• Publication Date: 2017-02-01
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c6cc09204g
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Two resorufin-based substrates were developed, providing sensitive fluorogenic readouts for PTP activity in vitro and in living cells. Abstract : Two resorufin-based substrates for protein tyrosine phosphatase (PTP) activity have been synthesized. These substrates provide sensitive fluorogenic readouts of PTP activity in vitro and in living cells at both acidic and neutral pH. In addition, the presence of the pathogenic bacteria Staphylococcus aureus was detected visually using a colorimetric readout.