Regulation of BC200 RNA‐mediated translation inhibition by hnRNP E1 and E2. Issue 2 (12th January 2017)

• Record Type: Journal Article
• Authors: Jang, Seonghui; Shin, Heegwon; Lee, Jungmin; Kim, Youngmi; Bak, Geunu; Lee, Younghoon
• Is Part Of: FEBS letters. Volume 591:Issue 2(2017)
• Page Start: 393
• Page End: 405
• Publication Date: 2017-01-12
• Subjects: BC200 RNA -- hnRNP E1 -- hnRNP E2 -- regulation of translation -- RNA‐protein interaction; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.12544
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : The long noncoding RNA BC200 (brain cytoplasmic RNA, 200 nucleotides) acts as a translational modulator of local protein synthesis at dendrites. BC200 RNA has been shown to inhibit translation in vitro, but it remains unknown how this translation inhibition might be controlled in a cell. Here, we performed yeast three‐hybrid screening and identified hnRNP E1 and hnRNP E2 as BC200 RNA‐interacting proteins. We found that: these hnRNA proteins could restore BC200 RNA‐inhibited translation; BC200 RNA interacts with hnRNP E1 and E2 mainly through its unique 3′ C‐rich domain; and the RNA binding specificities and modes of the two proteins differed somewhat. Our results offer new insights into the regulation of BC200 RNA‐mediated translation inhibition. Abstract :