SEC‐SANS: size exclusion chromatography combined in situ with small‐angle neutron scattering. Issue 6 (3rd November 2016)

• Record Type: Journal Article
• Authors: Jordan, Ashley; Jacques, Mark; Merrick, Catherine; Devos, Juliette; Forsyth, V. Trevor; Porcar, Lionel; Martel, Anne
• Is Part Of: Journal of applied crystallography. Volume 49:Issue 6(2016)
• Page Start: 2015
• Page End: 2020
• Publication Date: 2016-11-03
• Subjects: SEC‐SANS -- size exclusion chromatography -- small‐angle neutron scattering -- in situ -- sample environment; Crystallography -- Periodicals; 548.05
• DOI: 10.1107/S1600576716016514
• Languages: English
• ISSNs: 0021-8898

Abstract

Abstract : This publication describes the combination of size exclusion chromatography with small‐angle neutron scattering in situ . The need for this technique and the progress achieved thanks to it are illustrated by relevant examples. Abstract : The first implementation and use of an in situ size exclusion chromatography (SEC) system on a small‐angle neutron scattering instrument (SANS) is described. The possibility of deploying such a system for biological solution scattering at the Institut Laue–Langevin (ILL) has arisen from the fact that current day SANS instruments at ILL now allow datasets to be acquired using small sample volumes with exposure times that are often shorter than a minute. This capability is of particular importance for the study of unstable biological macromolecules where aggregation or denaturation issues are a major problem. The first use of SEC‐SANS on ILL's instrument D22 is described for a variety of proteins including one particularly aggregation‐prone system.