Optimising Terpene Synthesis with Flow Biocatalysis. Issue 2 (13th January 2017)

• Record Type: Journal Article
• Authors: Tang, Xiaoping; Allemann, Rudolf K.; Wirth, Thomas
• Is Part Of: European journal of organic chemistry. Issue 2(2017)
• Page Start: 414
• Page End: 418
• Publication Date: 2017-01-13
• Subjects: Biocatalysis -- Enzyme catalysis -- Flow chemistry -- Segmented flow -- Terpenoids -- Synthetic methods; Chemistry, Organic -- Periodicals; Organic compounds -- Synthesis -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1002/ejoc.201601388
• Languages: English
• ISSNs: 1434-193X

Abstract

Abstract : Sesquiterpenes are an important family of natural products, many of which exhibit important pharmaceutical and agricultural properties. They are biosynthesised from farnesyl diphosphate in sesquiterpene synthase catalysed reactions. Here, we report the development of a highly efficient segmented flow system for the enzyme‐catalysed continuous flow production of sesquiterpenes. Design of experiment (DoE) methods were used to optimise the performance of the flow biocatalysis, and quantitative yields were achieved by using an operationally simple but highly effective segmented flow system. Abstract : The application of segmented flow methodology in a simple flow reactor with purified enzymes as biocatalysts leads, after design of experiment (DoE) optimisation, to almost quantitative yields of terpenes.