A long wavelength fluorescent probe for biothiols and its application in cell imaging. Issue 37 (1st September 2016)

• Record Type: Journal Article
• Authors: Li, Jiawei; Yin, Caixia; Zhang, Yongbin; Chao, Jianbin; Huo, Fangjun
• Is Part Of: Analytical methods. Volume 8:Issue 37(2016)
• Page Start: 6748
• Page End: 6753
• Publication Date: 2016-09-01
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c6ay02150f
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : A long wavelength and turn-on fluorescent probe (1 ) containing the maleimide group as a reactive site was designed and synthesized. Abstract : A long wavelength and turn-on fluorescent probe (1 ) containing the maleimide group as a reactive site was designed and synthesized. A series of sensing studies showed that probe1 was highly selective and sensitive with fast responses towards bio-thiols over non-thiolated amino acids in DMSO : HEPES = 1 : 1 (v/v, pH 7.4) solution. Furthermore, the detection limit was as low as 0.015 μM. More importantly, the potential application of probe1 as a biosensor for thiols was demonstrated by employing it in fluorescence imaging of Cys in HepG2 cells.