Dynamic imaging of MYC and CDKN1A mRNAs as an indicator of cell G1-phase arrest. Issue 11 (24th January 2017)

• Record Type: Journal Article
• Authors: Yi, Linglu; Lin, Xuexia; Li, Haifang; Ma, Yuan; Lin, Jin-Ming
• Is Part Of: Chemical communications. Volume 53:Issue 11(2017)
• Page Start: 1900
• Page End: 1903
• Publication Date: 2017-01-24
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c6cc10078c
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A live-cell mRNA imaging method to dynamically identify cell transition stage G1-phase arrest. Abstract : The identification of G1-phase arrest requires a dynamic capturing method. In this work, we reported an indicator based on MYC and CDKN1A mRNA imaging to visualize G1-phase cycle arrest. A typical G1-phase arrest imaging is shown as a green-to-red conversion by fluorescence molecular beacons indicating the change as down-regulating of MYC and up-regulating of CDKN1A mRNA. We performed G1-phase arrest imaging on proliferated cancer cells inhibited by TMPyP4 and explore the role of HIF-1α in the proliferation inhibition effect.