Chemical probing of thiotetronate bio-assembly. Issue 11 (25th January 2017)

• Record Type: Journal Article
• Authors: Havemann, Judith; Yurkovich, Marie E.; Jenkins, Robert; Harringer, Sophia; Tao, Weixin; Wen, Shishi; Sun, Yuhui; Leadlay, Peter F.; Tosin, Manuela
• Is Part Of: Chemical communications. Volume 53:Issue 11(2017)
• Page Start: 1912
• Page End: 1915
• Publication Date: 2017-01-25
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c6cc09933e
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Chemical 'chain termination' probes were utilised for the investigation of thiotetronate antibiotic biosynthesis in the filamentous bacteria Lentzea sp. and Streptomyces thiolactonus NRRL 15439. Abstract : Chemical 'chain termination' probes were utilised for the investigation of thiotetronate antibiotic biosynthesis in the filamentous bacteria Lentzea sp. and Streptomyces thiolactonus NRRL 15439. The use of these tools led to the capture of biosynthetic intermediates involved in the thiotetronate polyketide backbone assembly, providing first insights into substrate specificity and in vivo intermediate processing by unusual iterative synthases.