Multiplexed detection of pathogen-specific DNA using engineered zinc finger proteins without target amplification. Issue 37 (2nd September 2016)

• Record Type: Journal Article
• Authors: Kim, Moon-Soo; Kim, Juhwa
• Is Part Of: Analytical methods. Volume 8:Issue 37(2016)
• Page Start: 6696
• Page End: 6700
• Publication Date: 2016-09-02
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c6ay02102f
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : Direct chemiluminescence detection of double-stranded DNA specific for bacterial pathogens using engineered zinc finger proteins (ZFPs). Abstract : Multiplexed detection of pathogen-specific DNA sequences in a simple and reliable way is in great demand for clinical and biomedical applications. However, there is still a lack of available DNA detection methods that are simple and pathogen-selective for point-of-care (POC) testing. Here, we report a novel zinc finger protein (ZFP)-based chemiluminescence method for direct detection of pathogenic double-stranded DNA (dsDNA) in a multiplexed platform. ZFPs are custom-designed to identify unique pathogenic DNA sequences. ZFP-based chemiluminescence detection of dsDNA provides sufficient sensitivity (≤50 fmol) and high specificity without target DNA amplification. Our study addresses the potential of developing a simple and selective pathogen detection method in a multiplexed fashion needed for POC applications.