Preventive effects of supplemental dietary zinc on heat-induced damage in the epididymis of boars. (February 2017)

• Record Type: Journal Article
• Authors: Li, Zhaojian; Li, Yansen; Zhou, Xin; Cao, Yun; Li, Chunmei
• Is Part Of: Journal of thermal biology. Volume 64(2016)
• Page Start: 58
• Page End: 66
• Publication Date: 2017-02
• Subjects: Heat stress -- Dietary zinc -- Epididymis -- Boars; Thermobiology -- Periodicals; Temperature -- Periodicals; Biology -- Periodicals; Thermobiologie -- Périodiques; Thermobiology; Periodicals; 571.46
• DOI: 10.1016/j.jtherbio.2017.01.002
• Languages: English
• ISSNs: 0306-4565

Abstract

Abstract: Hyperthermia in boars reduces growth performance and sperm production. Zinc is an essential trace element in animal nutrition. Here we investigate the effects of dietary zinc on epididymal structure and function in Bama miniature pigs treated with heat exposure and investigate approaches to improve the reproductive performance in summer. Male Bama miniature pigs (n=18; aged 6 months; bodyweight=10.79±0.06 kg) were randomly allocated to 3 groups: control group (Control), heat treatment group (HT), and the diet-supplemented and heat treatment group (H+Zn). The Control and HT groups were fed with basal diet and the H+Zn group were fed with basal diet plus 1500 mg/kg zinc daily. After being fed with these 2 different diets for 30 days, pigs in the HT and H+Zn groups were exposed to 5 h of 40 °C heat treatment for 8 days. Rectal temperature and jugular venous blood were collected 3 h after onset of heat exposure on days 1, 4 and 8. Pigs were sacrificed after the termination of heat exposure. Heat treatment increased serum testosterone concentration on day 1 and 4 ( P <0.01). In addition, the HT group displayed an increase in the clear cell count and a decrease in epithelium thickness in the caput epithelium ( P <0.01, P <0.05), and dietary zinc protected the boars from these impairments ( P <0.01, P =0.29). Evaluation of oxidative states showed that heat exposure increased the levels of malondialdehyde (MDA) and glutathione (GSH) in the epididymis ( P <0.01, P <0.05), while dietary zinc reduced this elevation ( P <0.01, P <0.01). Heat exposure enhanced the glucocorticoid receptor (GR) expression in the nuclei of principal and basal cells ( P <0.01, P <0.01) while dietary zinc attenuated the GR immunoreactivity intensity ( P <0.01, P <0.01). These results demonstrate that dietary zinc protects the epididymis from high temperature-induced impairment, alleviates oxidative stress, restores the integrity of the caput epithelium and decreases the stress response. Highlights: Dietary zinc diminished heat induced increase of serum testosterone content. Dietary zinc preserved the integrity of caput epididymis after heat exposure. Dietary zinc alleviated the stress condition in caput epididymis after heat exposure.