Mycophenolate mofetil attenuates uterine ischaemia/reperfusion injury in a rat model. Issue 2 (February 2017)

• Record Type: Journal Article
• Authors: Sahin Ersoy, Gulcin; Kurek Eken, Meryem; Cevik, Ozge; Cilingir, Ozlem T.; Tal, Reshef
• Is Part Of: Reproductive biomedicine online. Volume 34:Issue 2(2017)
• Page Start: 115
• Page End: 123
• Publication Date: 2017-02
• Subjects: mycophenolate mofetil -- oxygen species-free radicals -- rat -- uterine ischaemia-reperfusion -- uterine transplantation; Human reproductive technology -- Periodicals; Human embryo -- Periodicals; Reproduction -- Periodicals; 616.692
• DOI: 10.1016/j.rbmo.2016.11.007
• Languages: English
• ISSNs: 1472-6483

Abstract

Abstract: This study evaluated the effect of mycophenolate mofetil (MMF) on uterine tissue preservation following ischaemia/reperfusion (I/R) injury. Uterine I/R injury was induced in rats by clamping the lower abdominal aorta and ovarian arteries for 30 min. Group I/R + V ( n = 7) received vehicle alone while Group I/R + M ( n = 7) received 20 mg/kg/day MMF. Control groups underwent sham surgery and received vehicle (Group C) or 20 mg/kg/day MMF (Group M) ( n = 7 for both). Four hours after detorsion, uterine tissue 8-hydroxy-2′-deoxyguanosine (8-OHdG), glutathione, malondialdehyde (MDA), myeloperoxidase (MPO), superoxide dismutase (SOD) and serum ischaemia modified albumin (IMA) concentrations were measured. Histopathological analyses were performed. The I/R + M group showed significant reduction in serum IMA and uterine tissue 8-OHdG, MDA and MPO and significant increase in SOD concentrations compared with the I/R + V group, indicating a protective effect against I/R oxidative damage ( P = 0.009, P = 0.006, P = 0.002, P = 0.003 and P = 0.009, respectively). Histopathological evaluation revealed MMF treatment resulted in significantly less tissue and cellular damage and apoptosis compared with the I/R + V group. These results indicate MMF is effective in attenuating uterine tissue damage and preventing apoptosis following uterine I/R injury, probably via anti-inflammatory and anti-oxidative action.