Milk-derived tripeptides IPP (Ile-Pro-Pro) and VPP (Val-Pro-Pro) differentially modulate angiotensin II effects on vascular smooth muscle cells. (March 2017)

• Record Type: Journal Article
• Authors: Chakrabarti, Subhadeep; Liao, Wang; Davidge, Sandra T.; Wu, Jianping
• Is Part Of: Journal of functional foods. Volume 30(2017)
• Page Start: 151
• Page End: 158
• Publication Date: 2017-03
• Subjects: ACE angiotensin converting enzyme -- AngII angiotensin II -- BrDU bromodeoxyuridine -- DHE dihydroethidium -- ERK1/2 extracellular signal regulated kinases 1 and 2 -- IPP Ile-Pro-Pro -- NF-κB nuclear factor kappa B -- RAS renin-angiotensin system -- ROS reactive oxygen species -- VPP Val-Pro-Pro -- VSMC vascular smooth muscle cell; IPP -- VPP -- Lactotripeptides -- VSMC -- Inflammation -- Oxidative stress; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.12.022
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Lactotripeptides IPP and VPP were tested on vascular smooth muscle cells (VSMCs). VPP had anti-proliferative, anti-inflammatory and anti-oxidant actions on VSMC. VPP could potentially prevent vascular inflammation and remodeling in hypertension. Abstract: Hyperactivity of the Renin-Angiotensin System (RAS) is a hallmark of hypertension. Angiotensin II (AngII), the active component of RAS, not only promotes vasoconstriction but also contributes to proliferation, inflammation and oxidative stress in vascular smooth muscle cells (VSMC) that predispose to atherosclerosis. Milk derived bioactive peptides IPP (Ile-Pro-Pro) and VPP (Val-Pro-Pro) exert anti-hypertensive effects through RAS modulation. Here we evaluated the ability of these peptides to attenuate the effects of AngII on cultured VSMC. We found that VPP significantly inhibited AngII induced cell proliferation, inflammation and oxidative stress while IPP only showed anti-oxidant actions. Both peptides abolished AngII mediated activation of pro-inflammatory nuclear factor kappa B (NF-κB) pathway but only VPP could attenuate activation of extracellular signal regulated kinases (ERK) 1/2. These results demonstrate differential modulation of AngII actions on VSMC by anti-hypertensive milk peptides and indicate the potential for VPP in mitigating the vascular complications of hypertension.