The phytochemical composition, metabolites, bioavailability and in vivo antioxidant activity of Tetrastigma hemsleyanum leaves in rats. (March 2017)

• Record Type: Journal Article
• Authors: Sun, Yong; Tsao, Rong; Chen, Fang; Li, Hongyan; Wang, Jiawen; Peng, Han; Zhang, Ke; Deng, Zeyuan
• Is Part Of: Journal of functional foods. Volume 30(2017)
• Page Start: 179
• Page End: 193
• Publication Date: 2017-03
• Subjects: Tetrastigma hemsleyanum Leaf -- Phenolics -- Bioavailability -- Metabolism -- UPLC-ESI-QTOF-MS2 -- Antioxidant activity; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2017.01.004
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: A UPLC-ESI-QTOF-MS 2 method was used to identify the chemical constituents in THL. The metabolites in rats after administration THL extract were also investigated. The in vivo antioxidant activities of THL were evaluated for the first time. A total of 75 compounds were detected and 26 metabolites were identified. The phenolics of THL might play key role in the in vivo antioxidant activities. Abstract: Tetrastigma hemsleyanum is a traditional edible and medicinal plant in China. The phytochemical composition of its leaf (THL) and the bioavailability and metabolism of these compounds and their metabolites in vivo are less known. A metabolomics approach was therefore taken by using UPLC-ESI-QTOF-MS 2 . A total of 75 compounds were tentatively characterized in THL. Ten original compounds and 11 metabolites observed in plasma, and 20 original compounds and 26 metabolites detected in urine. Meanwhile, rats treated with THL extract in an oxidative stress model showed significantly increased total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities and glutathione (GSH) content and decreased malondialdehyde (MDA) content in plasma and tissues. Results suggest THL phenolics are bioavailable, and these compounds and their metabolites are likely behind the observed activities. The present study demonstrates that THL extract as a good source of antioxidant ingredient for the safeguard against oxidative stress-related chronic diseases.