Genotoxic and antiproliferative potential of extracts of Echinodorus grandiflorus and Sagittaria montevidensis (Alismataceae). Issue 1 (2nd January 2017)

• Record Type: Journal Article
• Authors: Coelho, Ana Paula Durand; Laughinghouse, Haywood Dail; Kuhn, Andrielle Wouters; Boligon, Aline Augusti; Canto-Dorow, Thais Scotti do; Silva, Antonio Carlos Ferreira da; Tedesco, Solange Bosio
• Is Part Of: Caryologia. Volume 70:Issue 1(2017)
• Page Start: 82
• Page End: 91
• Publication Date: 2017-01-02
• Subjects: Medicinal plants -- HPLC-DAD -- antimutagenic -- flavonoids -- Allium cepa -- mitotic index; Cell nuclei -- Periodicals; Cytogenetics -- Periodicals; Cytology -- Periodicals; 571.66
• DOI: 10.1080/00087114.2016.1275932
• Languages: English
• ISSNs: 0008-7114

Abstract

Abstract: The economic potential of the germplasm of medicinal species is a rich resource that should be preserved and used. The medicinal species Echinodorus grandiflorus and Sagittaria montevidensis belong to the family Alismataceae. Depurative, diuretic, anti-inflammatory, antivenin, and anti-rheumatic actions have been associated with E. grandiflorus, where the leaves are the main part used. Sagittaria montevidensis does not yet have its medicinal properties known and has a wide morphological variability, and is sometimes confused with E. grandiflorus . The present study aimed to evaluate the genotoxic and antiproliferative potential of extracts of dry leaves of E. grandiflorus and S. montevidensis on the cell cycle of Allium cepa, as well as undertake analysis by high performance liquid chromatography (HPLC-DAD) for the quantification of the compounds from the extracts. A. cepa bulb rootlets were submitted to the extracts for 24 h, prepared from plant material at two concentrations, 6 g dm −3 and 24 g dm −3 for each species, and two controls. Afterwards, the analysis of cell division and calculation of the mitotic index was undertaken. Part of the extracts was analyzed by HPLC-DAD. The results differed significantly between the controls and the treatments of both groups; the species did not have antimutagenic activity. The phytochemical analysis presented higher levels of caffeic acid and flavonoid glycoside in E. grandiflorus and phenolic glycoside in S. montevidensis. This study showed that the extracts of E. grandiflorus and S. montevidensis contain genotoxic potential and when used in high concentrations, anti-proliferative potential. HPLC-DAD revealed flavonoids and phenolic acids for both species.