Conversion of an instantaneous activating K+ channel into a slow activating inward rectifier. Issue 2 (2nd January 2017)

• Record Type: Journal Article
• Authors: Baumeister, Dirk; Hertel, Brigitte; Schroeder, Indra; Gazzarrini, Sabrina; Kast, Stefan M.; Van Etten, James L.; Moroni, Anna; Thiel, Gerhard
• Is Part Of: FEBS letters. Volume 591:Issue 2(2017)
• Page Start: 295
• Page End: 303
• Publication Date: 2017-01-02
• Subjects: inward rectification -- Kcv K+ channel -- salt bridges -- voltage‐dependent gating; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.12536
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : The miniature channel, Kcv, is a structural equivalent of the pore of all K + channels. Here, we follow up on a previous observation that a largely voltage‐insensitive channel can be converted into a slow activating inward rectifier after extending the outer transmembrane domain by one Ala. This gain of rectification can be rationalized by dynamic salt bridges at the cytosolic entrance to the channel; opening is favored by voltage‐sensitive formation of salt bridges and counteracted by their disruption. Such latent voltage sensitivity in the pore could be relevant for the understanding of voltage gating in complex Kv channels. Abstract :