Mycobacterium smegmatis proteoliposome induce protection in a murine progressive pulmonary tuberculosis model. (December 2016)

• Record Type: Journal Article
• Authors: Tirado, Yanely; Puig, Alina; Alvarez, Nadine; Borrero, Reinier; Aguilar, Alicia; Camacho, Frank; Reyes, Fatima; Fernandez, Sonsire; Perez, Jose Luis; Acevedo, Reynaldo; Mata Espinoza, Dulce; Payan, Jorge Alberto Barrios; Garcia, Maria de los A.; Kadir, Ramlah; Sarmiento, María E.; Hernandez-Pando, Rogelio; Norazmi, Mohd-Nor; Acosta, Armando
• Is Part Of: Tuberculosis. Volume 101(2016)
• Page Start: 44
• Page End: 48
• Publication Date: 2016-12
• Subjects: Mycobacterium smegmatis -- Proteoliposomes -- Tuberculosis -- Vaccines -- Challenge; TB tuberculosis -- Mtb Mycobacterium tuberculosis -- PL proteoliposomes -- BCG Bacillus Calmette–Guerin -- CFU Colony Forming Unit -- PLMs proteoliposomes obtained from Mycobacterium smegmatis -- PLMsAL PLMs adjuvanted with alum -- OMV outer membrane vesicles; 616.995
• DOI: 10.1016/j.tube.2016.07.017
• Languages: English
• ISSNs: 1472-9792

Abstract

Summary: Tuberculosis (TB) remains an important cause of mortality and morbidity. The TB vaccine, BCG, is not fully protective against the adult form of the disease and is unable to prevent its transmission although it is still useful against severe childhood TB. Hence, the search for new vaccines is of great interest. In a previous study, we have shown that proteoliposomes obtained from Mycobacterium smegmatis (PLMs) induced cross reactive humoral and cellular response against Mycobacterium tuberculosis (Mtb) antigens. With the objective to evaluate the protective capability of PLMs, a murine model of progressive pulmonary TB was used. Animals immunized with PLMs with and without alum (PLMs/PLMsAL respectively) showed protection compared to non-immunized animals. Mice immunized with PLMsAL induced similar protection as that of BCG. Animals immunized with BCG, PLMs and PLMsAL showed a significant decrease in tissue damage (percentage of pneumonic area/lung) compared to non-immunized animals, with a more prominent effect in BCG vaccinated mice. The protective effect of the administration of PLMs in mice supports its future evaluation as experimental vaccine candidate against Mtb.