Synthesis, characterization and DNA binding studies of two Ru(II) complexes containing guanidinium ligands. (28th May 2016)

• Record Type: Journal Article
• Authors: Chen, Wen-Xiu; Song, Xing-Dong; Chen, Jia-Xi; Zhao, Xuan-Hao; Xing, Jie-Hua; Ren, Jun-Rui; Wu, Tie; Sun, Jing
• Is Part Of: Polyhedron. Volume 110(2016)
• Page Start: 274
• Page End: 281
• Publication Date: 2016-05-28
• Subjects: Synthesis -- Ru(II) complex -- G-quadruplex DNA -- Guanidinium -- H-telo; Chemistry, Inorganic -- Periodicals; Chimie inorganique -- Périodiques; Organometaalverbindingen; Anorganische chemie; 546.05
• DOI: 10.1016/j.poly.2016.02.038
• Languages: English
• ISSNs: 0277-5387

Abstract

Graphical abstract: Two new Ru(II) complexes, [Ru(bpy)2 L] 3+ (1 and2 ), containing guanidinium ligands have been synthesized and characterized. Both complexes show good selectivity for h-telo G-quadruplex DNA compared to promoters and duplex DNA. Abstract: Two new Ru(II) complexes containing guanidinium groups have been synthesized, characterized and analyzed according to their interactions with different G-quadruplexes and duplex DNA. A FRET assay and a competitive FRET assay showed that both complexes promote the formation and stabilization of the human telomeric (h-telo) G-quadruplex and exhibit higher selectivity compared to promoters (such as c-myc, c-kit and bcl2) or duplex DNA. After binding to G-quadruplex, the two complexes have shown different DNA affinity and fluorescence enhancement. CD analyses further indicate that the two complexes display the ability to induce and stabilize the formation of antiparallel G-quadruplex structures in K +, Na + or ion-free buffers. The binding stoichiometry with h-telo was of the order of three ruthenium complexes per quadruplex.