A new drug delivery system for Mitomycin C to improve intravesical instillation. (15th November 2016)

• Record Type: Journal Article
• Authors: Sun, Xiaoliang; Sun, Peng; Li, Bo; Liu, Yiqing; Wang, Muwen; Suo, Ning; Yang, Min; Zhang, Dong; Jin, Xunbo
• Is Part Of: Materials & design. Volume 110(2016)
• Page Start: 849
• Page End: 857
• Publication Date: 2016-11-15
• Subjects: Drug delivery system -- Mitomycin C -- Intravesical instillation -- In situ forming depot -- Sustained release -- Apoptosis; Materials -- Periodicals; Engineering design -- Periodicals; Matériaux -- Périodiques; Conception technique -- Périodiques; Electronic journals; 620.11
• DOI: 10.1016/j.matdes.2016.08.058
• Languages: English
• ISSNs: 0264-1275

Abstract

Abstract: The conventional administration of Mitomycin C (MMC) in intravesical instillations has its limitations. In this study, MMC was loaded onto an in situ forming depot consisting of chitosan (CS), β-glycerophosphate (GP) and Fe3 O4 magnetic nanoparticles (Fe3 O4 -MNPs) to improve its effects. The features and effects of this new drug delivery mode were investigated. The new drug delivery system (Fe3 O4 -MMC-CS/GP) exhibited superior sol-gel transformation and magnetism. Sustained release of MMC was observed both in vitro and in vivo, and the retention, which lasted for 72 h in the rat bladder, was further examined using frozen sections. The Cell-Counting Kit-8 (CCK-8) and flow cytometry assays revealed better anti-tumor activity of Fe3 O4 -MMC-CS/GP compared with the free MMC solution. Animal experiments showed that Fe3 O4 -MMC-CS/GP provided a favorable survival rate and inhibited the growth of bladder tumors. Moreover, this drug delivery system enhanced tumor cell apoptosis compared with the conventional route of MMC administration in rats. Graphical abstract: Highlights: Mitomycin C was successfully loaded onto a new delivery system for intravesical instillation. The new delivery system exhibited excellent sustained release and prolonged retention properties. Both hematoxylin-eosin staining for retention and mimicry of urination were adequate and innovative experiment methods. The anti-tumor and apoptotic effect of Mitomycin C combined with the new delivery system was significantly enhanced.