The isomerase and hydratase reaction mechanism of the crotonase active site of the multifunctional enzyme (type‐1), as deduced from structures of complexes with 3S‐hydroxy‐acyl‐CoA. (15th February 2013)

• Record Type: Journal Article
• Authors: Kasaragod, Prasad; Schmitz, Werner; Hiltunen, Jukka K.; Wierenga, Rik K.
• Is Part Of: FEBS journal. Volume 280:Number 13(2013)
• Page Start: 3160
• Page End: 3175
• Publication Date: 2013-02-15
• Subjects: 3S‐hydroxy‐acyl‐CoA -- crotonase -- hydratase -- isomerase -- oxyanion hole; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pathology, Molecular -- Periodicals; 572
• DOI: 10.1111/febs.12150
• Languages: English
• ISSNs: 1742-464X

Abstract

Abstract : The multifunctional enzyme, type‐1 (MFE1) is involved in several lipid metabolizing pathways. It catalyses: (a) enoyl‐CoA isomerase and (b) enoyl‐CoA hydratase (EC 4.2.1.17) reactions in its N‐terminal crotonase part, as well as (3) a 3S‐hydroxy‐acyl‐CoA dehydrogenase (HAD; EC 1.1.1.35) reaction in its C‐terminal 3S‐hydroxy‐acyl‐CoA dehydrogenase part. Crystallographic binding studies with rat peroxisomal MFE1, using unbranched and branched 2E‐enoyl‐CoA substrate molecules, show that the substrate has been hydrated by the enzyme in the crystal and that the product, 3S‐hydroxy‐acyl‐CoA, remains bound in the crotonase active site. The fatty acid tail points into an exit tunnel shaped by loop‐2. The thioester oxygen is bound in the classical oxyanion hole of the crotonase fold, stabilizing the enolate reaction intermediate. The structural data of these enzyme product complexes suggest that the catalytic base, Glu123, initiates the isomerase reaction by abstracting the C2‐proton from the substrate molecule. Subsequently, in the hydratase reaction, Glu123 completes the catalytic cycle by reprotonating the C2 atom. A catalytic water, bound between the OE1‐atoms of the two catalytic glutamates, Glu103 and Glu123, plays an important role in the enoyl‐CoA isomerase and the enoyl‐CoA hydratase reaction mechanism of MFE1. The structural variability of loop‐2 between MFE1 and its monofunctional homologues correlates with differences in the respective substrate preferences and catalytic rates. Database: The structures have been deposited in the Protein Data Bank under accession numbers: 3ZW8 (MFE1 apo), 3ZW9 (MFE1 2S‐methyl‐3S‐hydroxy‐butanoyl‐CoA complex), 3ZWA (MFE1 3S‐hydroxy‐hexanoyl‐CoA complex), 3ZWB (MFE1‐E123A 2E‐hexenoyl‐CoA complex) and 3ZWC (MFE1 3S‐hydroxy‐decanoyl‐CoA complex). Structured digital abstract: MFE1 and MFE1 bind by x-ray crystallography (View interaction) Abstract : Protein crystallographic studies of multifunctional enzyme, type‐1 (MFE1) are reported. MFE1 catalyses two reactions of the β‐oxidation pathway, being a hydratase active site (provided by the crotonase domain) and a dehydrogenase active site (provided by the HAD domain). The studies focus on the catalytic properties of the hydratase active site, elucidating the mode of binding of the hydrated product molecules.