Validation of the Microreader™ 23sp ID system: A new STR 23-plex system for forensic application. (March 2017)

• Record Type: Journal Article
• Authors: Li, Jienan; Luo, Haibo; Song, Feng; Zhang, Lushun; Deng, Chuncao; Yu, Zailiang; Gao, Tianzhen; Liao, Miao; Hou, Yiping
• Is Part Of: Forensic science international. Volume 27(2017:Mar.)
• Page Start: 67
• Page End: 73
• Publication Date: 2017-03
• Subjects: Forensic science -- Validation -- Microreader™ 23sp ID system -- Short tandem repeat (STR); Forensic genetics -- Periodicals; Génétique légale -- Périodiques; Forensic genetics; Electronic journals; Periodicals; 614.1
• DOI: 10.1016/j.fsigen.2016.12.005
• Languages: English
• ISSNs: 1872-4973

Abstract

Highlights: A new 23-plex STR system contained 21 non-CODIS STR loci was validated. Applicable for 10 ul even 5 ul reaction volumes. Owned wide range of annealing temperatures with 58 °C to 62 °C. Abstract: Microreader™ 23sp ID system is a new 23-plex STR genotyping system that amplified 21 non-CODIS STR loci (D6S477, D18S535, D19S253, D15S659, D11S2368, D20S470, D1S1656, D22-GATA198B05, D7S3048, D8S1132, D4S2366, D21S1270, D13S325, D9S925, D3S3045, D14S608, D10S1435, D12S391, D2S1338, D17S1290 and D5S2500), one CODIS STR locus (D16S539) and the amelogenin locus in one reaction. Microreader™ 23sp ID system was validated according to the guidelines of "Validation Guidelines for DNA Analysis Methods (2012)" described by the Scientific Working Group on DNA Analysis Methods (SWGDAM), including PCR-based studies, sensitivity study, precision and accuracy evaluation, stutter percentage and peak height ratio, inhibitors, species specificity and DNA mixture studies. Our results suggested that Microreader™ 23sp ID system is a useful tool for identification and parentage testing.