Salmonella Enteritidis flagellar mutants have a colonization benefit in the chicken oviduct. (February 2017)

• Record Type: Journal Article
• Authors: Kilroy, Sofie; Raspoet, Ruth; Martel, An; Bosseler, Leslie; Appia-Ayme, Corinne; Thompson, Arthur; Haesebrouck, Freddy; Ducatelle, Richard; Van Immerseel, Filip
• Is Part Of: Comparative immunology, microbiology and infectious diseases. Volume 50(2017)
• Page Start: 23
• Page End: 28
• Publication Date: 2017-02
• Subjects: Salmonella -- Enteritidis -- Flagellin -- Laying hen -- Oviduct; Communicable diseases in animals -- Periodicals; Veterinary immunology -- Periodicals; Veterinary microbiology -- Periodicals; Immunology -- Periodicals; Microbiology -- Periodicals; Communicable diseases -- Periodicals; Communicable Diseases -- immunology -- Periodicals; Communicable Diseases -- veterinary -- Periodicals; Allergy and Immunology -- Periodicals; Microbiology -- Periodicals; Veterinary Medicine -- Periodicals; Immunologie -- Périodiques; Microbiologie -- Périodiques; Maladies infectieuses -- Périodiques; Communicable diseases; Immunology; Microbiology; Electronic journals; Periodicals; 636.08969
• DOI: 10.1016/j.cimid.2016.11.002
• Languages: English
• ISSNs: 0147-9571

Abstract

Highlights: Aflagellated S. Enteritidis have a colonization benefit in the oviduct in vivo. . S. Enteritidis downregulates flagellar gene transcription in chicken oviduct cells. Flagella are important for motility but not for invasion of oviduct cells. Aflagellated S. Enteritidis induces less inflammation in the chicken oviduct. Results indicate flagellin downregulation by S. Enteritidis to avoid immune response. Abstract: Egg borne Salmonella Enteritidis is still a major cause of human food poisoning. Eggs can become internally contaminated following colonization of the hen's oviduct. In this paper we aimed to analyze the role of flagella of Salmonella Enteritidis in colonization of the hen's oviduct. Using a transposon library screen we showed that mutants lacking functional flagella are significantly more efficient in colonizing the hen's oviduct in vivo . A micro-array analysis proved that transcription of a number of flagellar genes is down-regulated inside chicken oviduct cells. Flagella contain flagellin, a pathogen associated molecular pattern known to bind to Toll-like receptor 5, activating a pro-inflammatory cascade. In vitro tests using primary oviduct cells showed that flagellin is not involved in invasion. Using a ligated loop model, a diminished inflammatory reaction was seen in the oviduct resulting from injection of an aflagellated mutant compared to the wild-type. It is hypothesized that Salmonella Enteritidis downregulates flagellar gene expression in the oviduct and consequently prevents a flagellin-induced inflammatory response, thereby increasing its oviduct colonization efficiency.