Cyclin‐dependent kinase activity enhances phosphatidylcholine biosynthesis in Arabidopsis by repressing phosphatidic acid phosphohydrolase activity. (1st December 2016)

• Record Type: Journal Article
• Authors: Craddock, Christian P.; Adams, Nicolette; Kroon, Johan T.M.; Bryant, Fiona M.; Hussey, Patrick J.; Kurup, Smita; Eastmond, Peter J.
• Is Part Of: Plant journal. Volume 89:Number 1(2017:Jan.)
• Page Start: 3
• Page End: 14
• Publication Date: 2016-12-01
• Subjects: Arabidopsis thaliana -- phosphatidylcholine -- phospholipid biosynthesis -- membrane biogenesis -- phosphatidic acid phosphohydrolase -- phosphorylation -- cell cycle -- cyclin‐dependent kinase; Plant molecular biology -- Periodicals; Plant cells and tissues -- Periodicals; Botany -- Periodicals; 580
• DOI: 10.1111/tpj.13321
• Languages: English
• ISSNs: 0960-7412

Abstract

Significance Statement: Coordinating endomembrane biogenesis as cells progress thorough the cell cycle is important. Here we uncover a CYCLIN‐DEPENDENT KINASE A;1 regulatory mechanism that couples the rate of phospholipid biosynthesis and endomembrane biogenesis. Summary: Coordination of endomembrane biogenesis with cell cycle progression is considered to be important in maintaining cell function during growth and development. We previously showed that the disruption of PHOSPHATIDIC ACID PHOSPHOHYDROLASE (PAH) activity in Arabidopsis thaliana stimulates biosynthesis of the major phospholipid phosphatidylcholine (PC) and causes expansion of the endoplasmic reticulum. Here we show that PC biosynthesis is repressed by disruption of the core cell cycle regulator CYCLIN‐DEPENDENT KINASE A;1 (CDKA;1) and that this repression is reliant on PAH. Furthermore, we show that cyclin‐dependent kinases (CDKs) phosphorylate PAH1 at serine 162, which reduces both its activity and membrane association. Expression of a CDK‐insensitive version of PAH1 with a serine 162 to alanine substitution represses PC biosynthesis and also reduces the rate of cell division in early leaf development. Together our findings reveal a physiologically important mechanism that couples the rate of phospholipid biosynthesis and endomembrane biogenesis to cell cycle progression in Arabidopsis.