Glucose repression of FLO11 gene expression regulates pellicle formation by a wild pellicle-forming yeast strain isolated from contaminated wine. Issue 1 (2nd January 2017)

• Record Type: Journal Article
• Authors: Nakagawa, Youji; Arai, Yukari; Toda, Yasuhiro; Yamamura, Hideki; Okuda, Tohru; Hayakawa, Masayuki; Iimura, Yuzuru
• Is Part Of: Biotechnology, biotechnological equipment. Volume 31:Issue 1(2017)
• Page Start: 120
• Page End: 127
• Publication Date: 2017-01-02
• Subjects: Pellicle formation -- wild yeast -- Saccharomyces cerevisiae -- contaminated wine -- FLO11 expression -- glucose repression; Biotechnology -- Periodicals; Biotechnology -- Periodicals; Biotechnology -- instrumentation -- Periodicals; Periodicals; 660.605
• DOI: 10.1080/13102818.2016.1246203
• Languages: English
• ISSNs: 1310-2818

Abstract

ABSTRACT: Some wild strains of Saccharomyces cerevisiae form a pellicle on the surface of contaminated wines during the post-fermentation period of wine making. In this study, we found that both pellicle formation and FLO11 expression by a wild pellicle-forming strain of S. cerevisiae isolated from contaminated wine were repressed in a glucose-containing medium. Substitution of the promoter region of FLO11 in the cells with a constitutive promoter caused derepression of pellicle formation in the glucose-containing medium and wine. These findings indicate that glucose repression of the expression of the FLO11 gene, but not the glucose repression of the expression of other glucose-repressed genes, is responsible for the glucose-dependent regulation of pellicle formation by the wild pellicle-forming yeast strain. Furthermore, we found that the wild pellicle-forming strain had the same deletion of the 111-bp repression sequence in the FLO11 promoter as the flor strains commercially used for making sherry-like wines. Based on the results obtained in this study, a new method that would prevent deleterious pellicle formation in wineries is discussed.