A light and reduction dual sensitive supramolecular self-assembly gene delivery system based on poly(cyclodextrin) and disulfide-containing azobenzene-terminated branched polycations. Issue 47 (21st November 2016)

• Record Type: Journal Article
• Authors: Jiang, Qimin; Zhang, Yunti; Zhuo, Renxi; Jiang, Xulin
• Is Part Of: Journal of materials chemistry. Volume 4:Issue 47(2016)
• Page Start: 7731
• Page End: 7740
• Publication Date: 2016-11-21
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c6tb02248k
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : Light and reduction sensitive supramolecular host–guest gene vectors can regulate gene release upon exposure to reduction environments and light radiation inside cells. Abstract : Novel reduction degradable and photosensitive disulfide-containing azobenzene-terminated branched poly(2-(dimethylamino)ethyl methacrylate)s (Az-ss-BPDMs) and supramolecular host–guest self-assembly systems with poly(cyclodextrin) (PCD) were prepared and evaluated as non-viral gene delivery vectors. The reduction and light dual sensitive properties of the supramolecular polycations PCD/Az-ss-BPDMs and their polyplexes PCD/Az-ss-BPDMs/DNA were confirmed by UV-Vis, SEC, DLS and zeta potential analyses, respectively. It was shown that the inclusion of PCD, introduction of disulfide bonds into branched polycations, increase of the branching degree of the branched Az-ss-BPDMs and use of UV irradiation could enhance the gene transfection efficiency and cellular internalization of the supramolecular disulfide-containing azobenzene-terminated branched polycationic polyplexes (PCD/Az-ss-BPDM/DNA). Importantly, the transfection efficiency of the light and reduction dual-sensitive supramolecular PCD/Az-ss-BPDM/DNA polyplexes achieved almost 10 times higher value than that of 25 kDa PEI control; whereas the cytotoxicity of the supramolecular polyplexes was lower than that of PEI control. Thus this light and reduction dual responsive supramolecular host–guest system containing azobenzene-terminated branched cationic polymers with disulfide bonds and PCD is a promising gene vector.