Osteoinduction by combining bone morphogenetic protein (BMP)-2 witha bioactive novel nanocomposite. Issue 7 (July 2012)

• Record Type: Journal Article
• Authors: Sharma, A.; Meyer, F.; Hyvonen, M.; Best, S. M.; Cameron, R. E.; Rushton, N.
• Is Part Of: Bone & joint research. Volume 1:Issue 7(2012)
• Page Start: 145
• Page End: 151
• Publication Date: 2012-07
• Subjects: Osteoinduction -- BMP-2 -- Nanocomposite -- Polymer -- Ceramics -- Tissue engineering -- Orthopaedics; Musculoskeletal system -- Periodicals; 573.705
• DOI: 10.1302/2046-3758.17.2000082
• Languages: English
• ISSNs: 2046-3758

Abstract

Abstract : Objectives: There is increasing application of bone morphogenetic proteins(BMPs) owing to their role in promoting fracture healing and bonefusion. However, an optimal delivery system has yet to be identified.The aims of this study were to synthesise bioactive BMP-2, combineit with a novel α-tricalcium phosphate/poly(D, L-lactide-co-glycolide)(α-TCP/PLGA) nanocomposite and study its release from the composite. Methods: BMP-2 was synthesised using an Escherichia coli expressionsystem and purified. In vitro bioactivity was confirmedusing C2C12 cells and an alkaline phosphatase assay. The modifiedsolution-evaporation method was used to fabricate α-TCP/PLGAnanocomposite and this was characterised using X-ray diffractionand scanning electron microscopy. Functionalisationof α-TCP/PLGA nanocomposite by adsorption of BMP-2 was performedand release of BMP-2 was characterised using an enzyme-linked immunosorbentassay (ELISA). Results: Alkaline phosphatase activity of C2C12 cells was increased bythe presence of all BMP-2/nanocomposite discs compared with thepresence of a blank disc (p = 0.0022), and increased with increasingincubation concentrations of BMP-2, showing successful adsorptionand bioactivity of BMP-2. A burst release profile was observed forBMP-2 from the nanocomposite. Conclusions: Functionalisation of α-TCP/PLGA with BMP-2 produced osteoinductionand was dose-dependent. This material therefore has potential applicationas an osteoinductive agent in regenerative medicine.