Studies on the effect of agitation and aeration for the improved protease production by bacillus licheniformis NCIM-2042. (2016)

• Record Type: Journal Article
• Authors: Dey, Apurba; Bhunia, Biswanath; Dutta, Subhasish
• Is Part Of: Materials today. Volume 3(2016) Part A
• Page Start: 3444
• Page End: 3449
• Publication Date: 2016
• Subjects: Protease -- Agitation -- Aeration; Materials science -- Congresses -- Periodicals; 620.1
• DOI: 10.1016/j.matpr.2016.10.026
• Languages: English
• ISSNs: 2214-7853

Abstract

Abstract: The present research work deals with the effect of agitation and aeration for protease production using Bacillus licheniformis NCIM-2042 . The experiments were carried out in a lab scale 2.2 l bioreactor with 2 l working volume at 37 °C for 84 hour. Determination of kL a was estimated by dynamic gassing out method. The value of kL a was found to be 41, 54 and 56 1/h at 1, 2 and 3 vvm of airflow rate respectively. The maximum protease production was found to be 382.46 U at optimum flow rate of 2 vvm and agitation speed of 180 rpm.