Structure and anticoagulant property of a sulfated polysaccharide isolated from the green seaweed Monostroma angicava. (1st March 2017)

• Record Type: Journal Article
• Authors: Li, Na; Liu, Xue; He, Xiaoxi; Wang, Shuyao; Cao, Sujian; Xia, Zheng; Xian, Huali; Qin, Ling; Mao, Wenjun
• Is Part Of: Carbohydrate polymers. Volume 159(2017)
• Page Start: 195
• Page End: 206
• Publication Date: 2017-03-01
• Subjects: Acetonitrile (PubChem CID: 6342) -- Barium rhodizonic acid (PubChem CID: 45048930) -- Dimethyl sulfoxide (PubChem CID: 679) -- d-Glucuronic acid (PubChem CID: 94715) -- Heparin (PubChem CID: 25244225) -- Iodomethane (PubChem CID: 6328) -- l-Phenyl-3-methyl-5-pyrazolone (PubChem CID: 90474051) -- Pullunan (PubChem CID: 92024139) -- Pyridine (PubChem CID: 1049) -- Rhamnose (PubChem CID: 25310); Monostroma angicava -- Sulfated polysaccharide -- Structure -- Anticoagulant activity -- Inhibition mechanism; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2016.12.013
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Sulfated polysaccharide PF2 was isolated from the green seaweed Monostroma angicava . PF2 is a rhamnan-type sulfated polysaccharide with specific rhamnopyranose units. PF2 possesses a high anticoagulant activity in vitro . PF2 is a strong thrombin inhibitor mediated by heparin cofactor II. PF2 could be a potential anticoagulant polysaccharide. Abstract: An anticoagulant-active polysaccharide PF2 was extracted with boiling water from the green seaweed Monostroma angicava, further purified by anion-exchange and size-exclusion chromatography. PF2 was a rhamnan-type sulfated polysaccharide with molecular weight of about 88.1 kDa. Results of chemical and spectroscopic analyses demonstrated that PF2 consisted of → 3)-α-l - R ha p -(1→ and →2)-α-l - R ha p -(1 → residues, with partially branches at C-2 of → 3)-α-l - R ha p -(1 → residues. Sulfate groups were substituted at C-3 of → 2)-α-l - R ha p -(1 → residues. The sulfated polysaccharide PF2 had a high anticoagulant action, and the mechanism of anticoagulant activity mediated by PF2 was mainly attributed to strong potentiation thrombin by heparin cofactor II. PF2 also exhibited weak effect on antithrombin-dependent thrombin or factor Xa inhibition. The fibrin(ogen)olytic activity and thrombolytic activity of PF2 were also evaluated. The investigation revealed that PF2 was a novel sulfated rhamnan differing from previously described sulfated polysaccharides from green seaweed and could be a potential anticoagulant polysaccharide.