Effects of milk casein hydrolyzate supplemented with phytosterols on hypertension and lipid profile in hypercholesterolemic hypertensive rats. (January 2017)

• Record Type: Journal Article
• Authors: Ferreira-Santos, Pedro; Carrón, Rosalía; Recio, Isidra; Sevilla, M. Ángeles; Montero, M. José
• Is Part Of: Journal of functional foods. Volume 28(2017)
• Page Start: 168
• Page End: 176
• Publication Date: 2017-01
• Subjects: Casein hydrolyzate -- Hypertension -- Hypercholesterolemia -- Phytosterols -- SHR; ACh acetylcholine -- AI atherogenic index -- ALT alanine transaminase -- AST aspartate transaminase -- BW body weight -- CVD cardiovascular diseases -- DDC ammonium diethyldithiocarbamate -- HDLc high density lipoprotein-cholesterol -- LDLc low density lipoprotein-cholesterol -- LH liver hypertrophy -- LVH left ventricular hypertrophy -- NADPH nicotinamide adenosine dinucleotide phosphate -- O2− superoxide anion -- PE phenylephrine -- RLU relative luminescence units -- SBP systolic blood pressure -- SEM standard error of the mean -- SNP sodium nitroprusside -- TBARS thiobarbituric acid reactive substances -- TC Total cholesterol -- TG triacylglycerols; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.11.020
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Casein hydrolyzate plus phytosterols reduced hypertension and hypercholesterolemia. The effects on vascular reactivity and cardiac hypertrophy were due to hydrolyzate. Phytosterols were responsible for the lipid profile and liver damage improvements. The reduction of oxidative stress by hydrolyzate was increased by phytosterols. Abstract: This study investigates the beneficial effect of a milk casein hydrolyzate supplemented with phytosterols in hypercholesterolemic hypertensive rats. Spontaneously hypertensive rats were fed for 8 weeks with standard diet, or high cholesterol diet alone and supplemented with casein hydrolyzate or casein hydrolyzate plus phytosterols. Blood pressure was measured by tail-cuff method, vascular reactivity was performed on isolated aorta and biochemical parameters were evaluated in plasma. The hypercholesterolemic diet caused elevation of total cholesterol, low-density lipoprotein cholesterol, atherogenic index, lipid peroxidation, liver size and hepatic steatosis accompanied with a severe reduction of high-density lipoprotein cholesterol. The treatment with the casein hydrolyzate reduced blood pressure, cardiac hypertrophy and oxidative stress, and improved the endothelial function. The incorporation of phytosterols prevented the changes caused by the hypercholesterolemic diet and increased the antioxidant effect of casein hydrolyzate. In conclusion, treatment with milk casein hydrolyzate supplemented with phytosterols provides benefits when coexisting hypertension and hypercholesterolemia.