Garcinia kola aqueous suspension prevents cerebellar neurodegeneration in long-term diabetic rat – a type 1 diabetes mellitus model. (4th January 2017)

• Record Type: Journal Article
• Authors: Farahna, Mohammed; Seke Etet, Paul F.; Osman, Sayed Y.; Yurt, Kıymet K.; Amir, Naheed; Vecchio, Lorella; Aydin, Isınsu; Aldebasi, Yousef H.; Sheikh, Azimullah; Chijuka, John C.; Kaplan, Süleyman; Adem, Abdu
• Is Part Of: Journal of ethnopharmacology. Volume 195(2017)
• Page Start: 159
• Page End: 165
• Publication Date: 2017-01-04
• Subjects: CAST Computer Assisted Stereological Toolbox -- DAB 3, 3′-diaminobenzidine hydrochloride -- Fas Fas receptor -- i.p. intraperitoneal injection -- STZ streptozotocin -- TNF-α tumor necrosis factor; Streptozotocin (PubChem CID: 29327) -- D-Glucose (PubChem CID: 5793); Garcinia kola -- Neuroprotection -- Purkinje cell -- Type 1 diabetes mellitus -- Ataxia -- Medicinal plant; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2016.11.001
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: The development of compounds able to improve metabolic syndrome and mitigate complications caused by inappropriate glycemic control in type 1 diabetes mellitus is challenging. The medicinal plant with established hypoglycemic properties Garcinia kola Heckel might have the potential to mitigate diabetes mellitus metabolic syndrome and complications. Aim of the study: We have investigated the neuroprotective properties of a suspension of G. kola seeds in long-term type 1 diabetes mellitus rat model. Materials and methods: Wistar rats, made diabetic by single injection of streptozotocin were monitored for 8 months. Then, they were administered with distilled water or G. kola oral aqueous suspension daily for 30 days. Body weight and glycemia were determined before and after treatment. After sacrifice, cerebella were dissected out and processed for stereological quantification of Purkinje cells. Histopathological and immunohistochemical analyses of markers of neuroinflammation and neurodegeneration were performed. Results: Purkinje cell counts were significantly increased, and histopathological signs of apoptosis and neuroinflammation decreased, in diabetic animals treated with G. kola compared to diabetic rats given distilled water. Glycemia was also markedly improved and body weight restored to non-diabetic control values, following G. kola treatment. Conclusions: These results suggest that G. kola treatment improved the general condition of long-term diabetic rats and protected Purkinje cells partly by improving the systemic glycemia and mitigating neuroinflammation. Graphical abstract: