Enzymatic production of protein hydrolysates from steelhead (Oncorhynchus mykiss) skin gelatin as inhibitors of dipeptidyl-peptidase IV and angiotensin-I converting enzyme. (January 2017)

• Record Type: Journal Article
• Authors: Cheung, Imelda W.Y.; Li-Chan, Eunice C.Y.
• Is Part Of: Journal of functional foods. Volume 28(2017)
• Page Start: 254
• Page End: 264
• Publication Date: 2017-01
• Subjects: Gelatin hydrolysate -- Enzymatic hydrolysis -- Dipeptidyl-peptidase IV inhibitory activity -- Angiotensin-I converting enzyme inhibitory activity -- Ultrafiltration; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.10.030
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Gelatin hydrolysates with ACE and DPP-IV inhibitory activity were produced. Bioactivity was enhanced by either ultrafiltration or sequential enzyme hydrolysis. The <3 kDa fraction of hydrolysates produced by pepsin, Corolase®N or papain showed strong ACE and DPP-IV inhibition. ACE inhibitory activity of some hydrolysates improved after second hydrolysis with CorolaseN. Hydrolysis with papain followed by either ultrafiltration or CorolaseN yielded strong dual bioactivity. Abstract: The objective of this study was to generate protein hydrolysates with dual in vitro inhibitory activities against dipeptidyl-peptidase IV (DPP-IV) and angiotensin-I converting enzyme (ACE), by proteolytic enzymes acting either individually or sequentially on steelhead skin gelatin. The results showed strong dual bioactivity in the low molecular weight (<3 kDa) fractions obtained by ultrafiltration of hydrolysates produced using the enzymes pepsin, CorolaseN or papain. Alternatively, unfractionated hydrolysates exhibiting high ACE and DPP-IV inhibitory activities as well as yields could be attained by strategic selection of two enzymes for successive hydrolysis of gelatin. In particular, hydrolysates produced using 4% papain for 2 h followed either by ultrafiltration or by a second hydrolysis with 1% CorolaseN for 2 h, presented potent dual activity as ACE and DPP-IV inhibitors, and should be investigated further as potential functional food ingredients or nutraceuticals for the management of hypertension and diabetes.