Antioxidant and immunostimulating activities in vitro of sulfated polysaccharides isolated from Gracilaria rubra. (January 2017)

• Record Type: Journal Article
• Authors: Di, Tong; Chen, Guijie; Sun, Yi; Ou, Shiyi; Zeng, Xiaoxiong; Ye, Hong
• Is Part Of: Journal of functional foods. Volume 28(2017)
• Page Start: 64
• Page End: 75
• Publication Date: 2017-01
• Subjects: Gracilaria rubra -- Polysaccharide -- Characterization -- Antioxidant activity -- Immunostimulating activity; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.11.005
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Three polysaccharides (GRPS) were obtained from Gracilaria rubra by hot water. GRPS-1-1, GRPS-2-1 and GRPS-3-2 were all sulfated polysaccharides. GRPS-2-1 and GRPS-3-2 had little toxicity to L-02 cells. GRPS-3-2 exhibited excellent antioxidant and immunostimulating activities in vitro . Abstract: In this work, crude polysaccharides from Gracilaria rubra (GRPS) and its three purified fractions, GRPS-1-1, GRPS-2-1 and GRPS-3-2, were obtained through hot water extraction and chromatographic purification with DEAE-52 cellulose column and Sephadex G-50 column. The three purified fractions, being homogeneous heteropolysaccharides with average molecular weights of 1310, 691, 923 kD and sulfuric radical contents of 5.96, 8.46 and 12.03%, respectively, were all composed of galactose and fucose. GRPS-2-1 and GRPS-3-2 had little toxicity to L-02 cells. Crude GRPS and its purified fractions exhibited strong scavenging activities against ABTS and superoxide radicals and lipid peroxidation inhibition. At a cellular level, GRPS-3-2 showed the strongest protective effect on H2 O2 -induced oxidative injury in PC12 cells. Moreover, GRPS-3-2 exhibited a much stronger immunostimulating activity on RAW264.7 cells than GRPS-1-1 and GRPS-2-1. The results suggested that GRPS, especially GRPS-3-2, could be developed as a promising functional food supplement.