Effects of transient receptor potential canonical 1 (TRPC1) on the mechanical stretch-induced expression of airway remodeling-associated factors in human bronchial epithelioid cells. (25th January 2017)

• Record Type: Journal Article
• Authors: Yu, Qian; Li, Minchao
• Is Part Of: Journal of biomechanics. Volume 51(2017)
• Page Start: 89
• Page End: 96
• Publication Date: 2017-01-25
• Subjects: IL-13 interleukin-13 -- MMP-9 matrix metalloprotein-9 -- HBE human bronchial epithelioid -- TRPC1 transient receptor potential canonical 1 -- COPD chronic obstructive pulmonary disease -- PIP2 phosphatidylinositol-4, 5-bisphosphate -- PKC protein kinase C -- ATCC American Type Culture Collection -- EDTA Ethylene Diamine Tetraacetic Acid BAPTA-AM, 1, 2-Bis(2-aminophenoxy)ethane-N, N, N′, N′-tetraacetic acid tetrakis(acetoxymethyl ester) -- PVDF polyvinylidene fluoride -- SDS-PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis -- FCM flow cytometry TRPA1, transient receptor potential ankyrin 1 TG, trigeminal ganglion TRPM4, transient receptor potential melastain 4 TRPV1, transient receptor potential vanilloid 1 -- GPCRs G protein-coupled receptors -- PLCβ phospholipase Cβ -- IP3 inositol trisphosphate -- DAG diacylglycerol -- BAL bronchoalveolar lavage; Mechanical stretch -- Human bronchial epithelioid cells -- Interleukin-13 -- Matrix metalloprotein-9 -- Transient receptor potential canonical 1; Animal mechanics -- Periodicals; Biomechanics -- Periodicals; Biomechanics -- Periodicals; Mécanique animale -- Périodiques; Biomécanique -- Périodiques; Electronic journals; 571.4305
• DOI: 10.1016/j.jbiomech.2016.12.002
• Languages: English
• ISSNs: 0021-9290

Abstract

Abstract: Research has shown that mechanical stress stimulation can cause airway remodeling. We investigate the effects of mechanical stretch on the expression of the airway remodeling-associated factors interleukin-13 (IL-13) and matrix metalloprotein-9 (MMP-9) and signaling pathways in human bronchial epithelioid (16HBE) cells under mechanical stretch. A Flexcell FX-4000 Tension System with a flexible substrate was applied to stretch 16HBE cells at a 15% elongation amplitude and 1 Hz frequency, with stretching for 0.5 h, 1 h, 1.5 h and 2 h. The experimental group with higher IL-13, MMP-9, and TRPC1 expression and higher Ca 2+ levels was selected for performing intervention experiment. These cells were pretreated with the transient receptor potential canonical 1 (TRPC1) channel antagonist SKF96365 and TRPC1-specific siRNA, and then mechanical stretch was applied. Our results provided evidences that mechanical pressure significantly increased IL-13, MMP-9, and TRPC1 protein and mRNA expression levels and intracellular Ca 2+ fluorescence intensity at 4 time points compared with the control group. The peak IL-13, MMP-9, and TRPC1 expression levels were observed at 0.5 h after exposure to mechanical pressure. IL-13 and MMP-9 expression levels and Ca 2+ fluorescence intensity in the stretch+SKF96365 group and in the stretch+TRPC1 siRNA group were significantly lower than those were in the mechanical stretch group. By incubating the cells with the intracellular calcium chelator BAPTA-AM, the expression of IL-13 and MMP9 was significantly decreased, and the expression level of TRPC1 remained unchanged. These observations suggest that mechanical stretch may induce an influx of Ca 2+ and up-regulation of IL-13 and MMP-9 expression in 16HBE cells via activation of TRPC1.