Dietary live yeast and mannan-oligosaccharide supplementation attenuate intestinal inflammation and barrier dysfunction induced by Escherichia coli in broilers. Issue 11 (19th December 2016)

• Record Type: Journal Article
• Authors: Wang, Weiwei; Li, Zhui; Han, Qiqi; Guo, Yuming; Zhang, Bo; D'inca, Romain
• Is Part Of: British journal of nutrition. Volume 116:Issue 11(2016)
• Page Start: 1878
• Page End: 1888
• Publication Date: 2016-12-19
• Subjects: Live yeast, -- Mannan-oligosaccharide, -- Broilers, -- Intestinal inflammation, -- Intestinal barrier dysfunction; Nutrition -- Periodicals; 572.4
• DOI: 10.1017/S0007114516004116
• Languages: English
• ISSNs: 0007-1145

Abstract

Abstract: The effects of live yeast (LY) and mannan-oligosaccharide (MOS) supplementation on intestinal disruption induced by Escherichia coli in broilers were investigated. The experimental design was a 3×2 factorial arrangement with three dietary treatments (control, 0·5 g/kg LY ( Saccharomyces cerevisiae, 1·0×10 10 colony-forming units/g), 0·5 g/kg MOS) and two immune treatments (with or without E. coli challenge from 7 to 11 d of age). Samples were collected at 14 d of age. The results showed that E. coli challenge impaired ( P <0·05) growth performance during the grower period (1–21 d) and the overall period (1–35 d) of broilers, increased ( P <0·05) serum endotoxin and diamine oxidase levels coupled with ileal myeloperoxidase and lysozyme activities, whereas reduced ( P <0·05) maltase activity, and compromised the morphological structure of the ileum. Besides, it increased ( P <0·05) the mRNA expressions of several inflammatory genes and reduced occludin expression in the ileum. Dietary treatment with both LY and MOS reduced ( P <0·05) serum diamine oxidase and ileal myeloperoxidase levels, but elevated villus height ( P <0·10) and the ratio of villus height:crypt depth ( P <0·05) of the ileum. It also alleviated ( P <0·05) E. coli -induced increases ( P <0·05) in ileal Toll-like receptor 4, NF - κ B and IL-1 β expressions. Moreover, LY supplementation reduced ( P <0·05) feed conversion ratio of birds during the grower period and enhanced ( P <0·05) the community diversity (Shannon and Simpson indices) of ileal microbiota, whereas MOS addition counteracted ( P <0·05) the decreased ileal IL-10 and occludin expressions in challenged birds. In conclusion, both LY and MOS supplementation could attenuate E. coli -induced intestinal disruption by alleviating intestinal inflammation and barrier dysfunction in broilers. Moreover, LY addition could improve intestinal microbial community structure and feed efficiency of broilers.