Association of virulence factors, phylogenetic groups and antimicrobial resistance markers in Escherichia coli from Badin city, Pakistan. (2nd January 2017)

• Record Type: Journal Article
• Authors: Kumar, Neeraj; Nahid, Fouzia; Zahra, Rabaab
• Is Part Of: Journal of chemotherapy. Volume 29:Number 1(2017)
• Page Start: 8
• Page End: 13
• Publication Date: 2017-01-02
• Subjects: E. coli -- Phylogroups -- Virulence factors -- UTI -- NDM-1 -- CTX-M -- ESBL -- Pakistan; Chemotherapy -- Periodicals; Drug Therapy -- Periodicals; 615.58
• DOI: 10.1080/1120009X.2016.1154682
• Languages: English
• ISSNs: 1120-009X

Abstract

Abstract : Background : Escherichia coli, the most frequent cause of UTIs has extensive genetic substructure and can be assigned to eight phylogroups, A, B1, B2, C, D, E, F and Escherichia cryptic clade I. We investigated the distribution of virulence determinants and antimicrobial resistance genes in relation to phylogenetic groups. Methods : A total of 77 E. coli isolates were collected from Civil Hospital Badin, Pakistan. Isolates were assigned phylogroups using quadruplex PCR method, while virulence and antibiotic resistance genes, bla CTX-M and bla NDM-1 were also detected using PCR. Results : Thirty-four isolates were assigned to group B2, while 23, 2, 1, 7 and 10 isolates were assigned to F, B1, A/C, clade I/II and negative, respectively. Among virulence genes, prevalence of papC (83%) was highest followed by aer (57%), papG II (16%), papG III (14%), cnf (9%), hly (5%) and sfa (6%). Of these isolates, 23% and 9% were positive for bla CTX-M and bla NDM-1, respectively.