Ethanol Induces Platelet Apoptosis. (12th January 2017)

• Record Type: Journal Article
• Authors: Liu, Lei; Chen, Mengxing; Zhao, Lili; Zhao, Qing; Hu, Renping; Zhu, Jie; Yan, Rong; Dai, Kesheng
• Is Part Of: Alcoholism. Volume 41:Number 2(2017)
• Page Start: 291
• Page End: 298
• Publication Date: 2017-01-12
• Subjects: Ethanol -- Platelet -- Apoptosis; Alcoholism -- Periodicals; Alcoholism -- Periodicals; Alcoolisme; Electronic journals; Périodique électronique (Descripteur de forme); Ressource Internet (Descripteur de forme); 616.861005
• DOI: 10.1111/acer.13295
• Languages: English
• ISSNs: 0145-6008

Abstract

Abstract : Background: Alcohol abuse incurs severe medical conditions, such as thrombocytopenia and hemorrhage, but the pathogenesis is not totally understood. Alcohol has been reported to induce apoptosis in eukaryotic cells, such as hepatocyte, nerve cell, corneal fibroblasts. However, it is still unclear whether alcohol induces platelet apoptosis. Methods: Washed human platelets were pretreated with ethanol (EtOH), and apoptotic events and platelet function were detected. In in vivo experiments, C57BL/6J mice were given EtOH by gavage. Platelet counts, tail bleeding time, and the stomach were examined. Results: EtOH dose dependently induces depolarization of mitochondrial inner transmembrane potential, up‐regulation of Bax, down‐regulation of Bcl‐2, and caspase‐3 activation. EtOH does not induce surface expression of P‐selectin or PAC‐1 binding, whereas significantly reduces collagen‐, thrombin‐, and ADP‐induced platelet aggregation. Moreover, EtOH induces c‐Jun NH2‐terminal kinase activation. In an in vivo mouse model of the acute alcoholism, EtOH significantly reduces the number of circulating platelets, prolongs the tail bleeding time, and causes gastric mucosa hemorrhage. Conclusions: These data demonstrate that EtOH induces mitochondria‐mediated intrinsic platelet apoptosis, results in the reduction of the number of circulating platelets, and impairs in vivo hemostasis. These findings reveal the possible pathogenesis of hemorrhagic symptoms in patients experiencing acute alcohol intoxication.