Robust, universal biomarker assay to detect senescent cells in biological specimens. Issue 1 (17th November 2016)

• Record Type: Journal Article
• Authors: Evangelou, Konstantinos; Lougiakis, Nikolaos; Rizou, Sophia V.; Kotsinas, Athanassios; Kletsas, Dimitris; Muñoz‐Espín, Daniel; Kastrinakis, Nikolaos G; Pouli, Nicole; Marakos, Panagiotis; Townsend, Paul; Serrano, Manuel; Bartek, Jiri; Gorgoulis, Vassilis G.
• Is Part Of: Aging cell. Volume 16:Issue 1(2017)
• Page Start: 192
• Page End: 197
• Publication Date: 2016-11-17
• Subjects: aging -- biotin‐linked compounds -- immunohistochemistry -- senescence -- Sudan Black B; Cells -- Aging -- Periodicals; 571.8783605
• DOI: 10.1111/acel.12545
• Languages: English
• ISSNs: 1474-9718

Abstract

Summary: Cellular senescence contributes to organismal development, aging, and diverse pathologies, yet available assays to detect senescent cells remain unsatisfactory. Here, we designed and synthesized a lipophilic, biotin‐linked Sudan Black B (SBB) analogue suitable for sensitive and specific, antibody‐enhanced detection of lipofuscin‐containing senescent cells in any biological material. This new hybrid histo‐/immunochemical method is easy to perform, reliable, and universally applicable to assess senescence in biomedicine, from cancer research to gerontology.