Polyurethane/Polylactide-Based Electrospun Nonwovens as Carriers for Human Adipose-Derived Stromal Stem Cells and Chondrogenic Progenitor Cells. (11th December 2016)

• Record Type: Journal Article
• Authors: Marycz, Krzysztof; Marędziak, Monika; Grzesiak, Jakub; Fryczkowski, Ryszard; Laska, Jadwiga
• Is Part Of: Polymer-plastics technology and engineering. Volume 55:Number 18(2017)
• Page Start: 1897
• Page End: 1907
• Publication Date: 2016-12-11
• Subjects: Chondrogenic progenitor cells -- electrospinning -- mesenchymal stem cells -- polylactide -- polyurethane; Polymers -- Periodicals; Polymerization -- Periodicals; Polymers -- Periodicals; Engineering -- Periodicals; Plastics -- Periodicals; 668.9
• DOI: 10.1080/03602559.2016.1163586
• Languages: English
• ISSNs: 0360-2559

Abstract

ABSTRACT: Articular cartilage dysfunctions are major cause of pain and disability and lead to serious health complications. Cell-based therapies are proposed as treatment methods for cartilage regeneration. In this study, we proposed polyurethane/poly(L-lactide- co -D, L-lactide)-based electrospun nonwovens as carriers for the delivery of human adipose-derived stromal stem cells. We found that 6:4 and 8:2 polyurethane/poly(L-lactide- co -D, L-lactide) initially enhance proliferative rate of human adipose-derived stromal stem cells, shorten their population doubling time, promote creation of functional chondrogenic nodules during chondrogenic differentiation, improve the collagen-2-to-collagen-1 protein ratio, and upregulate the expression of collagen-2 and aggrecan genes. GRAPHICAL ABSTRACT: